Characterization of Enriched cultures of Nitrifying bacteria from Black cotton soil of PurnaBasin

To arrest the increasing nitrate pollution in Purna river basin due to indiscriminate anthropogenic commercial nitrogenous chemical fertilisation, the regional study of soil microbial nitrification was found to be important and in this regard, the causal soil chemoautotrophs were attempted for enrichmentand characterisation from the black cotton soil by adopting standard logical microbiological techniques. The species of Nitrosomonasand Nitrobacterwere traced to be behind nitrosification and nitrification in this heavily fertilized soilzone. These soil s have shown higher nitrification potentials due to the presence of nitrifying bacteria. These bacteria were enriched in liquid broths and characterised morphologically and biochemically for confirmation. The related details are discussed herewith.