红霉素
酶
降级(电信)
化学
基质(水族馆)
固定化酶
废水
酶分析
生物降解
抗生素
微生物学
核化学
色谱法
组合化学
生物化学
有机化学
生物
环境工程
环境科学
生态学
电信
计算机科学
作者
Shensheng Ni,Chunyu Li,Wenfan Zhang,Dongze Niu,Junqiang Zhi,Chongqing Wang,Xingmei Jiang,Jianjun Ren
标识
DOI:10.1016/j.envres.2023.117023
摘要
Erythromycin, a commonly used macrolide antibiotic, plays a crucial role in both human medicine and animal husbandry. However, its abuse has led to residual presence in the environment, with problems such as the emergence of resistant bacteria and enrichment of resistance genes. These issues pose significant risks to human health. Thus far, there are no effective, environmentally friendly methods to manage this problem. Enzymes can specifically degrade erythromycin without causing other problems, but their unrecyclability and environmental vulnerability hinder large-scale application. Enzyme immobilization may help to solve these problems. This study used Cu-BTC, a synthetic metal-organic framework, to immobilize the erythromycin-degrading enzyme EreB. The loading temperature and enzyme quantity were optimized. The Cu-BTC and EreB@Cu-BTC were characterized by various methods to confirm the preparation of Cu-BTC and immobilization of EreB. The maximum enzyme loading capacity was 66.5 mg g−1. In terms of enzymatic properties, immobilized EreB had improved heat (25–45 °C) and alkaline (6.5–10) tolerance, along with greater affinity between the enzyme and its substrate; Km decreased from 438.49 to 372.30 mM. Recycling was also achieved; after 10 cycles, 57.12% of the enzyme activity was maintained. After composite degradation, the antibacterial activity of erythromycin-containing wastewater was examined; the results showed that the novel composite could completely inactivate erythromycin. In summary, Cu-BTC was an ideal carrier for immobilization of the enzyme EreB, and the EreB@Cu-BTC composite has good prospects for the treatment of erythromycin-containing wastewater.
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