金属有机骨架
化学
环境修复
水解酶
纳米技术
酶
环境科学
有机化学
材料科学
生物化学
污染
生物
生态学
吸附
作者
Xin Yuan,Xiaoling Wu,Jing Xiong,Binhang Yan,Ruichen Gao,Shuli Liu,Min‐Hua Zong,Jun Ge,Wen‐Yong Lou
标识
DOI:10.1038/s41467-023-41716-6
摘要
Enzymes achieve high catalytic activity with their elaborate arrangements of amino acid residues in confined optimized spaces. Nevertheless, when exposed to complicated environmental implementation scenarios, including high acidity, organic solvent and high ionic strength, enzymes exhibit low operational stability and poor activity. Here, we report a metal-organic frameworks (MOFs)-based artificial enzyme system via second coordination sphere engineering to achieve high hydrolytic activity under mild conditions. Experiments and theoretical calculations reveal that amide cleavage catalyzed by MOFs follows two distinct catalytic mechanisms, Lewis acid- and hydrogen bonding-mediated hydrolytic processes. The hydrogen bond formed in the secondary coordination sphere exhibits 11-fold higher hydrolytic activity than the Lewis acidic zinc ions. The MOFs exhibit satisfactory degradation performance of toxins and high stability under extreme working conditions, including complicated fermentation broth and high ethanol environments, and display broad substrate specificity. These findings hold great promise for designing artificial enzymes for environmental remediation.
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