纤维素酶
里氏木霉
葡萄糖苷酶
分泌物
生物
高尔基体
细胞生物学
内质网
核糖体生物发生
生物化学
基因
酶
核糖体
核糖核酸
作者
Ai–Ping Pang,Haiyan Wang,Yongsheng Luo,Funing Zhang,Fugen Wu,Zhihua Zhou,Lu Zhang,Fengming Lin
标识
DOI:10.1016/j.engmic.2023.100105
摘要
β-glucosidases play an important role in the synthesis of cellulase in fungi, but their molecular functions and mechanisms remain unknown. We found that the 10 putative β-glucosidases investigated in Trichoderma reesei facilitate cellulase production, with cel3j being the most crucial. Transcriptional analysis revealed that the most affected biological processes in △cel3j strain were cellulase synthesis, ribosome biogenesis, and RNA polymerases. Moreover, CEL3J was unconventionally transported through the endoplasmic reticulum, bypassing the Golgi apparatus, whereas cel3j overexpression altered cellulase secretion from conventional to unconventional, likely owing to the activated unconventional protein secretion pathway (UPS), as indicated by the upregulation of genes related to UPS. The mTORC1-GRASP55 signaling axis may modulate the unconventional secretion of CEL3J and cellulase. The transcriptional levels of genes associated with DNA replication, the cell cycle, and meiosis were noticeably affected by overexpressing cel3j. These data give new clues for exploring the roles of β-glucosidases and the molecular mechanisms of their unconventional secretion in fungi.
科研通智能强力驱动
Strongly Powered by AbleSci AI