胰蛋白酶
芘
荧光
化学
鱼精蛋白
色谱法
胰蛋白酶原
生物化学
酶
有机化学
肝素
量子力学
物理
作者
Taemin Park,Minwoo Han,Kirk S. Schanze,Seoung Ho Lee
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2023-05-26
卷期号:8 (7): 2591-2597
被引量:4
标识
DOI:10.1021/acssensors.3c00297
摘要
The determination of trypsin activity in human urine is important for evaluating pancreatic disease. We designed an effective fluorescence sensing strategy based on a self-assembled amphiphilic pyrene/protamine complex system that provides an amplified fluorescence response for highly sensitive and selective detection of trypsin. In aqueous solution, the functionalized pyrene formed fluorescent, π-extended aggregates inside micelles, which were effectively quenched by protamine (a trypsin substrate). However, this quenched fluorescence was very sensitively recovered by the trypsin's enzymatic reaction, and this was attributed to a marked reduction in enhanced exciton migration caused by protamine in π-delocalized pyrene aggregates. The devised sensing platform was successfully utilized to selectively and sensitively detect trypsin at very low concentrations (0.03-0.5 μg mL-1) in non-pretreated human urine and to screen for trypsin inhibitors at concentrations of 0.1-5.0 μg mL-1.
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