超氧化物歧化酶
丙二醛
油菜籽
生物
过氧化氢酶
活性氧
抗氧化剂
脯氨酸
褪黑素
芸苔属
生物化学
糖
基因表达
过氧化物酶
食品科学
植物
酶
园艺
基因
内分泌学
氨基酸
作者
Lei Yan,Hairong He,Ali Raza,Zeng Liu,Xiaoyu Ding,Guijuan Wang,Yan Lv,Yongqiang Cheng,Xiling Zou
出处
期刊:Plant Signaling & Behavior
[Informa]
日期:2022-10-07
卷期号:17 (1)
被引量:15
标识
DOI:10.1080/15592324.2022.2129289
摘要
Rapeseed (Brassica napus L.) is an important oilseed crop globally. However, its growth and production are significantly influenced by cold stress. To reveal the protective role of exogenous melatonin (MEL) in cold tolerance, rapeseed seedlings were pretreated with different concentrations of MEL before cold stress. The results indicated that the survival rate was increased significantly by the MEL pretreatment under cold stress. Seedlings pretreated with 0.01 g L-1 MEL were all survived and were used to analyze the physiological characteristics and the expression level of various genes related to cold tolerance. Under cold stress, exogenous MEL significantly increased the contents of proline, soluble sugar, and soluble protein; while the malondialdehyde content was decreased by exogenous MEL under cold stress. On the other hand, the activities of antioxidant defense enzymes such as catalase, peroxidase, and superoxide dismutase were also significantly enhanced. The results also showed that MEL treatment significantly upregulated the expression of Cu-SOD, COR6.6 (cold-regulated), COR15, and CBFs (C-repeat binding factor) genes under cold stress. It was suggested exogenous MEL improved the content of osmotic regulatory substances to maintain the balance of cellular osmotic potential under cold stress and improved the scavenging capacity of reactive oxygen species by strengthening the activity of antioxidant enzymes and the cold-related genes expression.
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