Transcriptome analysis of the diseased intervertebral disc tissue in patients with spinal tuberculosis

椎间盘 肺结核 转录组 人类遗传学 生物 计算生物学 生物信息学 病理 医学 解剖 遗传学 基因 基因表达
作者
Tian’en Xu,Wenjuan Fan,Cong Chen,Kai Feng,Xiaoyun Sheng,Hong Wang,Kai Yang,Bao Chen,Xu Wang,Yapeng Wang
出处
期刊:BMC Medical Genomics [BioMed Central]
卷期号:17 (1) 被引量:2
标识
DOI:10.1186/s12920-024-01981-w
摘要

To investigate the differential expression genes (DEGs) in spinal tuberculosis using transcriptomics, with the aim of identifying novel therapeutic targets and prognostic indicators for the clinical management of spinal tuberculosis. Patients who visited the Department of Orthopedics at the Second Hospital, Lanzhou University from January 2021 to May 2023 were enrolled. Based on the inclusion and exclusion criteria, there were 5 patients in the test group and 5 patients in the control group. Total RNA was extracted and paired-end sequencing was conducted on the sequencing platform. After processing the sequencing data with clean reads and annotating the reference genome, FPKM normalization and differential expression analysis were performed. The DEGs and long non-coding RNAs (LncRNAs) were analyzed for Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment. The cis-regulation of differentially expressed mRNAs (DE mRNAs) by LncRNAs was predicted and analyzed to establish a co-expression network. This study identified 2366 DEGs, with 974 genes significantly upregulated and 1392 genes significantly downregulated. The upregulated genes are associated with cytokine-cytokine receptor interactions, tuberculosis, and TNF-α signaling pathways, primarily enriched in biological processes such as immunity and inflammation. The downregulated genes are related to muscle development, contraction, fungal defense response, and collagen metabolism processes. Analysis of LncRNAs from bone tuberculosis RNA-seq data detected a total of 3652 LncRNAs, with 356 significantly upregulated and 184 significantly downregulated. Further analysis identified 311 significantly different LncRNAs that could cis-regulate 777 target genes, enriched in pathways such as muscle contraction, inflammatory response, and immune response, closely related to bone tuberculosis. There are 51 genes enriched in the immune response pathway regulated by cis-acting LncRNAs. LncRNAs that regulate immune response-related genes, such as upregulated RP11-451G4.2, RP11-701P16.5, AC079767.4, AC017002.1, LINC01094, CTA-384D8.35, and AC092484.1, as well as downregulated RP11-2C24.7, may serve as potential prognostic and therapeutic targets. The DE mRNAs and LncRNAs in spinal tuberculosis are both associated with immune regulatory pathways. These pathways promote or inhibit the tuberculosis infection and development at the mechanistic level and play an important role in the process of tuberculosis transferring to bone tissue.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
西瓜完成签到 ,获得积分10
1秒前
123完成签到 ,获得积分10
2秒前
4秒前
丝丢皮的完成签到 ,获得积分10
5秒前
reset完成签到 ,获得积分10
8秒前
mojito完成签到 ,获得积分10
9秒前
四叶草完成签到 ,获得积分10
10秒前
八分饱应助无奈的小松鼠采纳,获得10
10秒前
10秒前
八分饱应助无奈的小松鼠采纳,获得10
10秒前
八分饱应助无奈的小松鼠采纳,获得10
10秒前
八分饱应助无奈的小松鼠采纳,获得10
11秒前
八分饱应助无奈的小松鼠采纳,获得10
11秒前
11秒前
八分饱应助无奈的小松鼠采纳,获得10
11秒前
八分饱应助无奈的小松鼠采纳,获得10
11秒前
小二郎应助无奈的小松鼠采纳,获得10
11秒前
大方博涛完成签到,获得积分10
12秒前
zzl完成签到,获得积分10
12秒前
赵小胖完成签到,获得积分10
22秒前
rita_sun1969完成签到,获得积分10
30秒前
jerry完成签到,获得积分10
32秒前
在水一方应助jerry采纳,获得10
39秒前
呆橘完成签到 ,获得积分10
40秒前
八分饱应助无奈的小松鼠采纳,获得10
41秒前
41秒前
八分饱应助无奈的小松鼠采纳,获得10
41秒前
41秒前
八分饱应助无奈的小松鼠采纳,获得10
41秒前
八分饱应助无奈的小松鼠采纳,获得10
41秒前
八分饱应助无奈的小松鼠采纳,获得10
41秒前
八分饱应助无奈的小松鼠采纳,获得10
41秒前
23应助无奈的小松鼠采纳,获得30
41秒前
41秒前
万金油完成签到 ,获得积分10
45秒前
义气兔子发布了新的文献求助10
46秒前
51秒前
安静严青完成签到 ,获得积分10
53秒前
57秒前
CMD完成签到 ,获得积分10
57秒前
高分求助中
【重要!!请各位用户详细阅读此贴】科研通的精品贴汇总(请勿应助) 10000
Plutonium Handbook 1000
Three plays : drama 1000
International Code of Nomenclature for algae, fungi, and plants (Madrid Code) (Regnum Vegetabile) 1000
Semantics for Latin: An Introduction 999
Robot-supported joining of reinforcement textiles with one-sided sewing heads 580
Apiaceae Himalayenses. 2 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4092137
求助须知:如何正确求助?哪些是违规求助? 3630863
关于积分的说明 11507751
捐赠科研通 3341979
什么是DOI,文献DOI怎么找? 1836948
邀请新用户注册赠送积分活动 904840
科研通“疑难数据库(出版商)”最低求助积分说明 822585