化学
过氧化物酶
纳米棒
检出限
催化作用
锌
核化学
食腐动物
激进的
色谱法
酶
生物化学
有机化学
纳米技术
材料科学
作者
Yaru Liu,Pingping Hao,Zhenchao Liu,Guijiang Li,Gao-Chao Fan,Min Xie,Qingyun Liu
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:148 (2): 269-277
被引量:1
摘要
Exploring highly active peroxidase mimics at physiological pH is important for the construction of efficient and convenient colorimetric sensing platforms for detecting small biomolecules. In this work, prepared zinc pyrovanadate (Zn3V2O7(OH)2·2H2O) nanorods exhibit excellent peroxidase-like activity, which is verified by the fast oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) into a blue product (oxTMB) by H2O2 at physiological pH (pH = 7) in 2 min. In addition, the catalytic behaviors of Zn3V2O7(OH)2·2H2O as a peroxidase-like nanozyme conform to the Michaelis-Menten equation. Scavenger experiments prove that the catalytic activity of Zn3V2O7(OH)2·2H2O is ascribed to ˙O2- radicals generated in the process of catalysis. Based on the peroxidase-like activity of the Zn3V2O7(OH)2·2H2O nanozyme, a fast and convenient colorimetric sensor has been constructed to detect H2O2 and epinephrine (EP) under physiological pH. The detection limit of EP is as low as 0.26 μM. In addition, the feasibility of the proposed sensor has been validated to detect H2O2 in milk and EP in serum.
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