Rutin Ameliorates H2O2-Induced Oxidative Stress Injury in HaCaT Cells via the Nrf2-Regulated Pathway

Oxidative stress induced by harmful substances activates inflammatory signaling pathways and causes excessive proliferation of keratinocytes, which is related to the occurrence of psoriasis. Rutin, a natural citrus flavonoid glycoside, exhibits protective effects against oxidative stress. However, whether rutin is able to influence oxidative stress in keratinocytes remains unclear. In the present study, an in vitro cell model of human keratinocytes (HaCaT) treated with H2O2 was used to explore whether rutin can prevent oxidative stress. The present findings suggest that rutin protected HaCaT cells against oxidative damage by inhibiting ROS, NO and MDA secretion, increasing SOD activity and restoring GSH-Px activity. In addition, rutin supplement limited IL-6, IL-1β and IL-23A production in HaCaT cells. Moreover, rutin upregulated Nrf2 expression, and promoted the downstream NQO1 and HO-1 expression. These results suggest that rutin may inhibit HaCaT cell oxidative stress by modulating the Nrf2-regulated pathway.