蔗糖
己糖
果糖
糖
生物
蔗糖合成酶
生物化学
转录因子
碳水化合物代谢
基因表达
基因
植物
酶
转化酶
作者
Heting Fang,Yanna Shi,Shengchao Liu,Rong Jin,Jun Sun,Donald Grierson,Shaojia Li,Kunsong Chen
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2023-03-13
卷期号:192 (3): 1858-1876
被引量:19
标识
DOI:10.1093/plphys/kiad156
摘要
Abstract Sugars are fundamental to plant developmental processes. For fruits, the accumulation and proportion of sugars play crucial roles in the development of quality and attractiveness. In citrus (Citrus reticulata Blanco.), we found that the difference in sweetness between mature fruits of “Gongchuan” and its bud sport “Youliang” is related to hexose contents. Expression of a SuS (sucrose synthase) gene CitSUS5 and a SWEET (sugars will eventually be exported transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these 2 varieties, revealed higher expression levels in “Youliang” fruit. The roles of CitSUS5 and CitSWEET6 were investigated by enzyme activity and transient assays. CitSUS5 promoted the cleavage of sucrose to hexoses, and CitSWEET6 was identified as a fructose transporter. Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6. The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5 overexpression, -VIGS, and -RNAi. CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of “Youliang” and “Gongchuan” fruit.
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