毛状体
生物
植物
转录组
拟南芥
开枪
基因
基因表达
遗传学
突变体
作者
Peina Zhou,Hongyu Chen,Jingjie Dang,Zunrui Shi,Yongfang Shao,Chanchan Liu,Longjiang Fan,Qinan Wu
标识
DOI:10.3389/fpls.2022.988594
摘要
The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were used to isolated protoplasts and successfully captured 33,254 protoplasts for sequencing purposes. After cell type annotation, all the cells were partitioned into six broad populations with 19 clusters. Cells from PGTs were identified based on the expression patterns of trichome-specific genes, monoterpene biosynthetic genes, and metabolic analysis of PGT secretions. The developmental trajectories of PGTs were delineated by pseudotime analysis. Integrative analysis of scRNA-seq data from N. tenuifolia leaves and Arabidopsis thaliana shoot revealed that PGTs were specific to N. tenuifolia . Thus, our results provide a promising basis for exploring cell development and differentiation in plants, especially glandular trichome initiation and development.
科研通智能强力驱动
Strongly Powered by AbleSci AI