Resolving the Mutually Exclusive Immune Responses of Chitosan with Nanomechanics and Immunological Assays

Abstract Multifaceted functions displayed by both pro‐ and anti‐inflammatory properties of chitosan hinder its effective development as an immunomodulatory agent. Herein, the contributions of the bending stiffness of chitosan with regard to its immune regulatory properties toward inflammation are investigated. The anti‐inflammatory properties of chitosan molecular weight (MW) with a shorter (≈1 kDa) or longer (≈15 kDa) than the persistent length ( L P ) are compared using immunological assays and nanomechanics‐based experiments on the surface forces apparatus (SFA). Interestingly, 1 kDa chitosan significantly enhances the generation of anti‐inflammatory regulatory T cells (Tregs) through the Dectin‐1‐dependent pattern recognition receptor (PRR) on antigen‐presenting cells. SFA analyses also show a similar trend of interaction forces between chitosan and diverse PRRs depending on their MW. The results obtained in the immunological and nanomechanical experiments are consistent and imply that the binding features of PRRs vary depending on the MW of chitosan, which may alter immune activity. In accordance, in vivo administration of only 1 kDa represses inflammatory responses and suppresses the progression of experimental colitis. This study elucidates a previously unexplored bending stiffness‐dependent immune regulatory property of chitosan and suggests the applicability of low MW (rod‐like) chitosan as a pharmaceutical ingredient to treat diverse inflammatory disorders.