PLGA公司
牛血清白蛋白
纳米颗粒
荧光
生物物理学
体外
化学
材料科学
化学工程
纳米技术
色谱法
生物化学
生物
物理
量子力学
工程类
作者
Nicoletta Rescignano,Luigi Tarpani,A. Romani,Ilaria Bicchi,Simona Mattioli,Carla Emiliani,Luigi Torre,J. M. Kenny,Sabata Martino,Loredana Latterini,Ilaria Armentano
标识
DOI:10.1016/j.polymdegradstab.2016.10.017
摘要
The hydrolytic degradation of poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles loaded with bovine serum albumin (BSA) covalently labelled with a fluorescent dye (FITC) was investigated spectrally resolving the fluorescence signals. Studies have been conducted in-vitro in phosphate buffer saline and in human adult bone marrow-mesenchymal stem cell cultures. We demonstrated that PLGA nanoparticles degraded in heterogeneous manner and a particle agglomeration process occurs after 21 days of incubation owing to particle fragmentation and leading to cargo release. Additionally, we showed that degradation of up-taken FITC-BSA-PLGA nanoparticles by stem cells may be monitored by the emission spectrum of the released FITC-BSA that shifted towards shorter wavelengths and becomes narrower, after 230 h. Moreover, the changes of fluorescence spectra revealed that the loaded protein is released in the cells during polymer degradation.
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