赫普斯
特里斯
生物
人工海水
生物物理学
色谱法
植物
海水
生物化学
化学
生态学
作者
G. I. McFadden,Michael Melkonian
出处
期刊:Phycologia
[Informa]
日期:1986-12-01
卷期号:25 (4): 551-557
被引量:227
标识
DOI:10.2216/i0031-8884-25-4-551.1
摘要
N-2-hydroxyethylpiperazine-N-2′-ethanesulfonic acid (Hepes) has been tested as an alternative to tris-(hydroxymethyl)aminoethane (Tris) for hydrogen-ion buffering of algal cultures. Hepes satisfactorily buffered both freshwater and artificial seawater media and has been used successfully to grow 25 species of microalgae from five classes. Electron microscopic fixations of Tetraselmis striata Butcher (Prasinophyceae) and Amphidinium rhyncocephalum Anissimowa (Dinophyceae) buffered by Hepes have given far superior ultrastructural preservation than fixations buffered by Tris. The substitution of Hepes for Tris allows a defined seawater medium to be used as a fixative vehicle for the first time.
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