Liposomes from Novel Photolabile Phospholipids: Light-Induced Unloading of Small Molecules As Monitored by PFG NMR

A molecular dithiane-based approach to synthesis of novel photolabile phospholipids is developed. These lipids are used in formulations with egg-POPC and cholesterol to prepare light-sensitive liposomes. Irradiation of such liposomes in PBS buffer (medium pressure mercury lamp, Pyrex filter, λ > 300 nm) significantly increases the bilayer permeability and accelerates the release of entrapped small organic molecules by an order of magnitude. A simple assay, based on 1H or 19F PFG NMR measurements of diffusion coefficients, is develped to monitor light-induced unloading of the probe molecules.