脂质体
生物发光成像
癌症研究
荧光素酶
体内
药物输送
卵巢癌
癌基因
HER2/东北
靶向给药
癌症
化学
药理学
生物
细胞
细胞培养
医学
药品
转染
细胞周期
生物化学
内科学
生物技术
有机化学
乳腺癌
遗传学
作者
Xiao‐Jian Han,Yongfang Wei,Yu‐Ying Wan,Liping Jiang,Jianfeng Zhang,Hong‐Bo Xin
标识
DOI:10.3892/ijmm.2014.1922
摘要
Liposomes as targeted drug delivery systems are an emerging strategy in the treatment of cancer to selectively target tumors or genes. In this study, we generated the recombinant protein, EC1-GLuc, by fusing the EC1 peptide, an artificial ligand of ErbB2, with Gaussia luciferase (GLuc). The purified EC1-GLuc was conjugated with a nickel-chelating liposome to construct the EC1-GLuc-liposome. In vitro experiments revealed that the EC1-GLuc-liposome selectively targeted and internalized into ErbB2-overexpressing SKOv3 cells for bioluminescence imaging. A cell-impermeable fluorescence dye (HPTS) encapsulated in the EC-GLuc-liposome was efficiently delivered into the SKOv3 cells. In addition, the EC1-GLuc-liposome also targeted metastatic SKOv3 tumors for bioluminescence imaging and effectively delivered HPTS into metastatic tumors in vivo. Therefore, the present study demonstrates the novel EC1-GLuc-liposome to be an effective theranostic system for monitoring and treating ErbB2-overexpressing metastatic ovarian carcinoma through a combination of targeted molecular imaging and DDS.
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