RAC1
板层
生物
GTP酶
细胞迁移
RNA干扰
细胞生物学
癌变
癌症研究
小干扰RNA
细胞
细胞培养
信号转导
转染
核糖核酸
癌症
遗传学
基因
作者
Alice Chan,Salvatore J. Coniglio,Ya Yu Chuang,David Michaelson,Ulla G. Knaus,Mark R. Philips,Marc Symons
出处
期刊:Oncogene
[Springer Nature]
日期:2005-07-18
卷期号:24 (53): 7821-7829
被引量:225
标识
DOI:10.1038/sj.onc.1208909
摘要
Members of the Rho family of small GTPases have been shown to be involved in tumorigenesis and metastasis. Currently, most of the available information on the function of Rho proteins in malignant transformation is based on the use of dominant-negative mutants of these GTPases. The specificity of these dominant-negative mutants is limited however. In this study, we used small interfering RNA directed against either Rac1 or Rac3 to reduce their expression specifically. In line with observations using dominant-negative Rac1 in other cell types, we show that RNA interference-mediated depletion of Rac1 strongly inhibits lamellipodia formation, cell migration and invasion in SNB19 glioblastoma cells. Surprisingly however, Rac1 depletion has a much smaller inhibitory effect on SNB19 cell proliferation and survival. Interestingly, whereas depletion of Rac3 strongly inhibits SNB19 cell invasion, it does not affect lamellipodia formation and has only minor effects on cell migration and proliferation. Similar results were obtained in BT549 breast carcinoma cells. Thus, functional analysis of Rac1 and Rac3 using RNA interference reveals a critical role for these GTPases in the invasive behavior of glioma and breast carcinoma cells.
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