衣壳
二十面体对称
病毒
噬菌体
蛋白质结构
生物
生物物理学
计算生物学
物理
结晶学
化学
病毒学
遗传学
基因
生物化学
大肠杆菌
作者
Preeti Gipson,Matthew L. Baker,Desislava Raytcheva,Cameron Haase-Pettingell,Jacqueline Piret,Jonathan King,Wah Chiu
摘要
Marine viruses play crucial roles in shaping the dynamics of oceanic microbial communities and in the carbon cycle on Earth. Here we report a 4.7-Å structure of a cyanobacterial virus, Syn5, by electron cryo-microscopy and modelling. A Cα backbone trace of the major capsid protein (gp39) reveals a classic phage protein fold. In addition, two knob-like proteins protruding from the capsid surface are also observed. Using bioinformatics and structure analysis tools, these proteins are identified to correspond to gp55 and gp58 (each with two copies per asymmetric unit). The non 1:1 stoichiometric distribution of gp55/58 to gp39 breaks all expected local symmetries and leads to non-quasi-equivalence of the capsid subunits, suggesting a role in capsid stabilization. Such a structural arrangement has not yet been observed in any known virus structures.
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