生物
RAPD
遗传学
基因座(遗传学)
标记辅助选择
基因
遗传标记
裂解扩增多态序列
基因型
等位基因
遗传多样性
人口
限制性片段长度多态性
人口学
社会学
作者
Naweed I. Naqvi,Bharat B. Chattoo
出处
期刊:Genome
[Canadian Science Publishing]
日期:1996-02-01
卷期号:39 (1): 26-30
被引量:89
摘要
Two randomly amplified polymorphic DNA (RAPD) markers, OPF6(2700) and OPH18(2400), tightly linked to Pi-10, a dominant blast-resistance gene conferring complete resistance to isolate 106 (international race IB46) of the blast fungus were identified. To derive sequence characterized amplified regions (SCARs) from OPF6(2700) and OPH18(2400), these amplified RAPD products were cloned and sequenced. Nucleotide sequence information, obtained for each end of the two linked RAPD markers, was used to design 24-mer oligonucleotide primers for PCR amplification of the respective SCARs. Polymorphisms appearing as differences in the length of the SCAR's alternate alleles were considered for the indirect selection of Pi-10. Such polymorphisms converted the linked dominant RAPD loci into codominant SCAR markers and also facilitated the indirect scoring of the blast-resistant and blast-susceptible genotypes. The development of length variant codominant SCAR markers linked to a major gene for blast resistance in rice is described. The codominant SCARs will facilitate marker-assisted selection of the Pi-10 locus in rice breeding programs and will also be useful as genetic markers for high resolution mapping of the Pi-10 region.
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