岩藻糖基化
抗磷脂综合征
抗体
免疫学
糖基化
医学
免疫球蛋白G
同型
血栓形成
聚糖
自身抗体
狼疮抗凝剂
内科学
糖蛋白
分子生物学
生物
单克隆抗体
生物化学
作者
Tingting Liu,Jing Han,Rongrong Zhang,Zihan Tang,Gang Yi,Wen Gong,Liyan Wan,Qiongyi Hu,Jialin Teng,Honglei Liu,Xiaobing Cheng,Junna Ye,Yutong Su,Yue Sun,Yi Shi,Jianxin Gu,Shifang Ren,Chengde Yang,Hui Shi
出处
期刊:Rheumatology
[Oxford University Press]
日期:2021-05-20
卷期号:61 (3): 1243-1254
被引量:3
标识
DOI:10.1093/rheumatology/keab416
摘要
Anti-β-2 glycoprotein I (anti-β2GPI) antibodies, defined as primary pathogenic antibody in antiphospholipid syndrome (APS). It has been reported that IgG Fc N-glycosylation affects IgG effector, we aim to investigate the association of Fc glycosylation profiles of purified anti-β2GP1 IgG with clinical features of APS.We purify anti-β2GPI IgG and total IgG from 82 APS patients including nine catastrophic antiphospholipid syndrome (CAPS) patients, as well as total IgG from 103 healthy controls to quantitatively analyse all detectable Fc N-glycanforms of all IgG subclasses with Multiple Reaction Monitoring (MRM) method based on UPLC-ESI-QqQ mass spectrometry.Both purified anti-β2GPI IgG and APS total IgG showed altered N-glycan profiles when compared with healthy control (HC) IgG. Anti-β2GPI IgG presented with lower galactosylation, increased bisection and core fucosylation compared with APS total IgG and HC IgG. We found higher galactosylation of aβ2GPI IgG2 in thrombotic APS compared with the obstetric APS, and lower galactosylation of aβ2GPI IgG2 associated with late pregnancy morbidity. Moreover, low galactosylation of all anti-β2GPI IgG subclasses, increased bisection and core fucosylation of anti-β2GPI IgG1/2 were strongly associated with CAPS and triple positivity of antiphospholipid antibodies (aPLs).We comprehensively characterize the N-Glycans landscape of both anti-β2GP1 and total IgG in APS. Altered N-glycan profiles of anti-β2GPI IgG enables enabled the antibodies with proinflammatory properties. Furthermore, we associated levels of IgG Fc-glycosylation with clinical features antiphospholipid syndrome. These findings could increase our understanding of anti-β2GPI antibody mediated mechanisms in APS and be used to develop diagnostics and new target treatments.
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