锁孔血蓝蛋白
免疫系统
过继性细胞移植
刺激
免疫学
异硫氰酸荧光素
骨髓
树突状细胞
CD40
细胞因子
生物
获得性免疫系统
敏化
脂多糖
化学
内分泌学
T细胞
体外
细胞毒性T细胞
荧光
物理
量子力学
生物化学
作者
Georges J. M. Maestroni
标识
DOI:10.1016/s0165-5728(02)00188-1
摘要
The information gathered by dendritic cells (DC) during the innate immune response to a pathogen is determinant for the type of adaptive response. Here we show that short-term (3 h) exposure of bone marrow-derived DC to norepinephrine (NE), at the beginning of lipopolysaccharide (LPS) or keyhole limpet hemocyanin (KLH) stimulation hampers IL-12 production and increases IL-10 release. The NE effect was mediated by both beta- and alpha2-adrenergic receptors. The capacity of NE-exposed DC to produce IL-12 upon CD40 cross-linking as well as to stimulate allogeneic T-helper (Th) lymphocytes was reduced. Adoptive transfer of NE-exposed DC induced a Th2 slanted response in vivo. Thus, a brief NE exposure of antigen-stimulated DC seems to limit their Th1 polarizing properties. Noteworthy, the ganglionic blocker pentolinium administered in mice before skin sensitization with fluoroscein isothiocyanate (FITC) could increase the Th1-type response in the draining lymph nodes. Our results suggest that the extent of Th differentiation in the response to an antigen might be influenced by the local sympathetic nervous activity in the early phase of dendritic cell stimulation.
科研通智能强力驱动
Strongly Powered by AbleSci AI