癌症研究
效力
肝细胞癌
癌变
上皮-间质转换
癌症干细胞
转录因子
干细胞
转移
化学
医学
癌症
生物
内科学
基因
体外
细胞生物学
生物化学
作者
Dongmin Shi,Xiaoli Shi,Kailin Xing,Hongxin Zhou,Lili Lü,Wei‐Zhong Wu
标识
DOI:10.1016/j.cellsig.2020.109650
摘要
Epithelial–mesenchymal transition (EMT), a pivotal event during cancer progression such as relapse and metastasis, is positively correlated with the stemness potency of tumor cells. Our previous study showed that miR-296-5p attenuated EMT program of hepatocellular carcinoma cells (HCC) through NRG1/ERBB2/ERBB3 signaling. In the present study, we uncovered that miR-296-5p was able to inhibit the stemness potency of HCC by decreasing the number and size of tumorspheres, downregulating the expression of CSC biomarkers and hampering the ability of tumorigenesis in NOD/SCID mice. Brahma-related gene-1 (Brg1), as the target protein of miR-296-5p detected by bioinformatics methods, activates a series of downstream cascades through directly binding to Sall4 promoter and enhancing Sall4 transcription. Importantly, the higher expressions of Brg1 and Sall4 in tumor tissues of HCC patients suggest poorer prognoses after surgical extraction. In conclusion, miR-296-5p exerts an inhibitory effect on stemness potency of HCC cells via Brg1/Sall4 axis.
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