千分尺
聚吡咯
材料科学
静电纺丝
基质(水族馆)
纳米
雪旺细胞
生物物理学
发芽
纳米纤维
纳米技术
聚合物
高分子化学
聚合
解剖
复合材料
生物
物理
光学
植物
生态学
作者
Fernando Gisbert Roca,Jorge Más Estellés,Manuel Monleón Pradas,Cristina Martínez‐Ramos
标识
DOI:10.1016/j.ijbiomac.2020.09.181
摘要
The biological behaviour of Schwann cells (SCs) and dorsal root ganglia (DRG) on fibrillar, highly aligned and electroconductive substrates obtained by two different techniques is studied. Mats formed by nanometer-sized fibres of poly(lactic acid) (PLA) are obtained by the electrospinning technique, while bundles formed by micrometer-sized extruded PLA fibres are obtained by grouping microfibres together. Both types of substrates are coated with the electrically conductive polymer polypyrrole (PPy) and their morphological, physical and electrical characterization is carried out. SCs on micrometer-sized substrates show a higher motility and cell-cell interaction, while a higher cell-material interaction with a lower cell motility is observed for nanometer-sized substrates. This higher motility and cell-cell interaction of SCs on the micrometer-sized substrates entails a higher axonal growth from DRG, since the migration of SCs from the DRG body is accelerated and, therefore, the SCs tapestry needed for the axonal growth is formed earlier on the substrate. A higher length and area of the axons is observed for these micrometer-sized substrates, as well as a higher level of axonal sprouting when compared with the nanometer-sized ones. These substrates offer the possibility of being electrically stimulated in different tissue engineering applications of the nervous system.
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