Design, synthesis and antimicrobial activity of an hybrid antimicrobial peptide LFB15-MA12.

A novel hybrid antimicrobial peptide,LFB15-MA12,derived from the core function sequence of LfcinB and Magainin,was designed by connection between Lfcin B (1-15) and Magainin (1-12).The peptide LFB15-MA12 with purity above 95% was obtained by solid phase synthesis of chemical method.The antimicrobial activity of the peptide was determined by small quantity broth microdilution method and MIC for S.aureus ATCC25923,E.coli ATCC25922,P.aeruginosa ATCC 27853 and S.typhimurium C77-31 was 16,64,64 and 32 μg·mL-1,respectively.The hemolytic activity was not observed for the peptide at the range of inhibitory concentration.A novel antimicrobial peptide,with improved antibacterial activity and property of safety and nontoxicity was obtained.This study provided a new method for research and development of novel antimicrobial peptides with ideal activities.