纳米柱
生物传感器
多路复用
纳米技术
小RNA
化学
材料科学
计算生物学
生物信息学
基因
生物
生物化学
纳米结构
作者
Woo Hyun Kim,Jong Uk Lee,Sojin Song,Soohyun Kim,Young Shik Choi,Sang Jun Sim
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2019-01-01
卷期号:144 (5): 1768-1776
被引量:53
摘要
Cell-free micro-ribonucleic acids (cf-miRNAs) regulate most human genes by controlling the mRNA translation and have been considered as the most promising biomarkers for cancer diagnosis and prognosis. Due to their low abundance, short length, and similar sequences, an attractive approach that is ultrasensitive and label-free is highly demanded. Herein, we develop a label-free, ultra-high sensitivity and selectivity multiplex miRNA using surface-enhanced Raman scattering to detect cancer-associated miRNAs with head-flocked gold nanopillars as a substrate. Using a complementary DNA probe platform and an ultra-high sensitivity SERS based biosensor, we achieved high selectivity in targeting miRNAs with extremely low detection limits, without any chemical or enzymatic reactions and evaluated the correlation between the miRNA expression level and the Raman signal intensity. A reproducible SERS signal is monitored via uniformly fabricated elastic head-flocked gold nanopillars through a mapping method. This system supports the detection of cell-free miRNAs in blood, which are utilized and quantified as biomarkers to diagnose and provide a prognosis for cancers and intractable diseases. In the foreseeable future, this advanced label-free miRNA detection system is highly promising for a less tumor-invasive biopsy for the early diagnosis and prognosis of cancer.
科研通智能强力驱动
Strongly Powered by AbleSci AI