New species of Trichoderma in the Harzianum, Longibrachiatum and Viride clades

During field surveys in central and southwestern China, three new species, Trichoderma acremonioides, T. rugosum and T. subalni were discovered. They are located respectively in the Viride, Longibrachiatum and Harzianum clades. Trichoderma acremonioides from southwest of China forms reddish brown ascomata, hyaline ascospores and very simple conidiophores. Trichoderma rugosum occurring in Hubei and Tibet is specialized by stroma cortical tissue of textura intricata, hyaline ascospores, and trichoderma-like conidiophores. Trichoderma subalni from Tibet is characterized by rufous stromata, green ascospores, trichoderma-like conidiophores, and slow growing on standard media. Detailed morphological distinctions between the new species and their close relatives are discussed. Their phylogenetic positions were determined based on sequence analyses of RPB2 and EF-1α genes.