驯化
生物
基因组编辑
近亲繁殖
遗传学
基因
基因组
清脆的
生物技术
人口
社会学
人口学
作者
Tingdong Li,Xinping Yang,Yuan Yu,Xiaomin Si,Xiaoyan Zhai,Huawei Zhang,Wenxia Dong,Caixia Gao,Xu Cao
摘要
Crop improvement by inbreeding often results in fitness penalties and loss of genetic diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by using multiplex CRISPR-Cas9 editing of coding sequences, cis-regulatory regions or upstream open reading frames of genes associated with morphology, flower and fruit production, and ascorbic acid synthesis. Cas9-free progeny of edited plants had domesticated phenotypes yet retained parental disease resistance and salt tolerance.
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