生物
转录组
低温保存
单细胞分析
计算生物学
核糖核酸
细胞
细胞生物学
遗传学
基因表达
基因
胚胎
作者
Amy Guillaumet-Adkins,Gustavo Rodríguez-Esteban,Elisabetta Mereu,María Méndez-Lago,Diego Adhemar Jaitin,Alberto Villanueva,August Vidal,Alex Martinez-Martí,Enriqueta Felip,Ana Vivancos,Hadas Keren‐Shaul,Simon Heath,Marta Gut,Ido Amit,Ivo G. Gut,Holger Heyn
出处
期刊:Genome Biology
[Springer Nature]
日期:2017-03-01
卷期号:18 (1)
被引量:136
标识
DOI:10.1186/s13059-017-1171-9
摘要
A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.
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