Objective:To analyze the import of rotavirus infection in infantile diarrhea.Methods:Stool specimens from 1 323 infants with diarrhea from 2001 to 2003 were detected by ELISA.Results:Rotavirus was detected from 600 of 1 323 stool specimens.The positive rate was 45.4% in the years.The peak season of rotavirus infection was from October to December.The positive rates of the age groups of ≤6 months,6 months to 2 years,2 to 5 years were 18.8%,53.4% and 13.8% respectively in 2003.The positive rate of the males was higher than that of the females( P 0.05).Conclusions:Rotavirus was the main pathogen in infantile diarrhea of the age group of 6 months to 2 years.