PRDM5 Gene Methylation and Expression in HTB-182、A549

Objective: To detect PRDM5 high methylation state in HTB-182,A549,HBE normal bronchial epithelium in cell lines and demethylation treatment to influencing of the expression.Methods: After different concerntration of demethylation drug treatment in lung cancer cell lines(HTB-182,A549) Sand HBE(bronchial epithelium cells),as control without drugs,examined methylation status,expression of mRNA and protein of PRDM5 in lung cancer cell lines and HBE.Results: There were different degree of high methylation in HTB-182,A549.After adding to different concerntrations of demethylation drug treatment,the level of methylation reduced gradually,the expression of mRNA and protein increased gradually.Conclusions: PRDM5 promoter methylation is the direct reason of cell lines PRDM5 expression level of lowering.PRDM5 promoter methylation may be the new target of lung cancer treatment.