Determination of Calycosin-7-Glucoside in Astragalus membranaceus(Fisch.) Bunge by HPLC

The contents of calycosin-7-glucoside of Astragalus membranaceus(Fisch.) Bunge samples from different producing areas were determined by HPLC with calycosin-7-glucoside content as measurement quality index,and the medicinal materials quality standards of Astragalus membranaceus(Fisch.) Bunge was established,then the quality of Astragalus membranaceus(Fisch.) Bunge from the different producing areas was studied.The 68 samples were from seven provinces in China.The detection results of 68 Astragalus membranaceus(Fisch.) Bunge from 7 provinces showed the differences of calycosin-7-glucoside componential contents were large in Astragalus membranaceus(Fisch.) Bunge from different producing areas,and contents ordered as follows:ShanxiInner MongoliaGansuLiaoning.The differences of calycosin-7-glucoside componential contents were big among different varieties of Astragalus membranaceus(Fisch.) Bunge,but the contents of cultivar varieties were less than wild varieties.The contents of calycosin-7-glucoside componential were different due to different growing time,and contents of growing time law the order as follows:two yearsfive yearsthree yearsfour years.The quality evaluation of Astragalus membranaceus(Fisch.) Bunge by HPLC was available,and can provide the scientific and confident judgment for pharmacy factory in the selection of Astragalus membranaceus(Fisch.) Bunge.