Effect of T-2 toxin on integrins expression and antagonistic role of selenium

Objective To study the effects of T-2 toxin and selenium on integrins expression to explore the relationship of T-2 toxin and selenium with the etiology of Kashin-Beck disease.Methods We used MTT assay to assess the proliferation of chondrocytes C-28/I2 treated with different concentrations of T-2 toxin and select the optimal time and concentration of T-2 toxin.Meanwhile,Real-time PCR was used to analyze the expression of integrins treated wih T-2 toxin or selenium.Results T-2 toxin could inhibit the proliferation of chondrocytes C-28/I2.Integrins profile changed with the addition of T-2 toxin;the significantly upregulated integrins were αv and β1,while the downregulated integrins were α2,α5 and β5.Moreover,selenium could partly block the integrins changes.Conclusion T-2 toxin could obviously inhibit the growth of chondrocytes while selenium could protect chondrocytes by antagonizing the expression of integrins.