Determination of Catalpol in Leaves of Rehmannia glutinosa Libosch. by HPLC

Objective To determine the content of catalpol in the leaves of Rehmannia glutinosa Libosch..Methods Catalpol in leaves of Rehmannia glutinosa Libosch.was determinated by HPLC.The mobile phase was acetonitrile-0.1% phosphoricacid(1:99).The detection wavelength was set at 210 nm.The flow rate was 1.0 mL·min-1.The column temperature was controlled at 40℃.Results The content of catalpol in leaves of Rehmannia glutinosa Libosch.was 2.3%.Conclusion The content of catalpol in the leaves of Rehmannia glutinosa Libosch.is higher.The leaves of Rehmannia glutinosa Libosch.is worth developing.