Primary Culture and Identification of Human Folliole Granulosa Cells

Objective To establish and improve the primary culture in vitro and identification methods of human folliole granulosa cells.Methods Primary granulosa cells were obtained from women who were meeting the inclusion criteria and undergoing ART.Folliole granulosa cells were incubated in the DMEM medium.H-E staining and FSHR immunohistochemistry methods were used to identify folliole granulosa cells.Results Over 90% of the cultured cells were folliole granulosa cells.Cell purity was above 95% by FSHR immunohistochemical identification.Cultured human folliole granulosa cells during the first 2～5d were to reach the splitting peak.Conclusions Good growth activity,cell purity and more stable human folliole granulosa cells could be obtained by the method used in this study.Human folliole granulosa cells could be simply and rapidly identified by H-E staining and FSHR immunohistochemistry methods.