The liquid culture media of Cordyceps sinensis mycelia was optimized by means of orthogonal analysis.The results showed that the optimal medium for the mycelia was 1.25% glucose,1.25% sucrose,0.02% peptone,0.062 5% yeast powder,0.025% KH2PO4,0.012 5% MgSO4·7H2O,0.002 5% VB1 and pH natural.In the cultural condition of 24 ℃ and 192 h,the mycelia biomass reached to 19.5 g/L,which was as 1.2~1.5 times as the amount with the previous way.