维斯坎
蛋白质水解
蛋白多糖
化学
细胞外基质
阿达姆斯
细胞生物学
蛋白酵素
硫酸软骨蛋白多糖
硫酸软骨素
生物化学
生物
糖胺聚糖
金属蛋白酶
基质金属蛋白酶
血栓反应素
酶
作者
Simon J. Foulcer,Anthony J. Day,Suneel S. Apte
出处
期刊:Methods in molecular biology
日期:2021-10-10
卷期号:: 559-578
被引量:1
标识
DOI:10.1007/978-1-0716-1398-6_43
摘要
Versican is a widely distributed chondroitin sulfate proteoglycan that forms large complexes with the glycosaminoglycan hyaluronan (HA). As a consequence of HA binding to its receptor CD44 and interactions of the versican C-terminal globular (G3) domain with a variety of extracellular matrix proteins, versican is a key component of well-defined networks in pericellular matrix and extracellular matrix. Versican is crucial for several developmental processes in the embryo ranging from cardiac development to digit separation, and there is an increasing interest in its roles in cancer and inflammation. Versican proteolysis by ADAMTS proteases is highly regulated, occurs at specific peptide bonds, and is relevant to several physiological and disease mechanisms. In this chapter, methods are described for the isolation and detection of intact and cleaved versican in tissues using morphologic and biochemical techniques. These, together with the methodologies for purification and analysis of recombinant versican and an N-terminal versican fragment named versikine that are provided here, are likely to facilitate further progress on the biology of versican and its proteolysis.
科研通智能强力驱动
Strongly Powered by AbleSci AI