基因
生物
遗传学
序列分析
发起人
序列(生物学)
塔塔盒子
CAAT箱
反聚合酶链反应
完整序列
DNA测序
克隆(编程)
互补DNA
聚合酶链反应
基因组
基因表达
套式聚合酶链反应
计算机科学
程序设计语言
出处
期刊:Journal of Wuhan Botanical Research
日期:2009-01-01
摘要
TaPSG719 gene is a pollen-specific gene isolated from wheat and its function is remains unknown.The aims of the study were to isolate the TaPSG719 promoter sequence and to characterize the promoter regions responsible for tissue-specificity.A pair of special primers was designed according to the cDNA sequence of TaPSG719 gene reported.The upstream regulatory sequence was cloned from total DNA of wheat (Bobwhite) by inverse-PCR.A fragment of 1776 base pairs was obtained by two rounds of inverse-PCR.Sequence analysis of functional elements by blasting PLACE and PlantCARE databases revealed that it contains TATA-box,CAAT-box,two pollen-specific elements (AGAAA and GTGA),several light responsive elements and hormone responsive elements.Promoter analysis of this gene would definitely help us to understand its function as well as the molecular biology of wheat male gametophyte development.
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