Keratin-inorganic hybrid nanoflowers decorated with Fe3O4 nanoparticles as enzyme mimics for colorimetric detection of glucose

Fe3O4 magnetic nanoparticles (MNPs) are highly active enzyme-like catalysts. However, low stability is still a big challenge for Fe3O4-based enzyme mimics because the Fe3O4 MNPs can be easily dissolved when exposed to acidic conditions. Inspired by the numerous catalytic sites of a flower-like structure and the biological functions of amino acids in structural proteins, herein, by employing keratin as a protein component, stable Fe3O4-based MNP embedded keratin-Cu3(PO4)2 nanoflowers were constructed, from which hierarchical nanostructures with a three-dimensional petal-like morphology were selected for subsequent studies owing to their excellent enzymic catalytic activity. The keratin-nanoflower@Fe3O4 exhibited significantly enhanced catalytic activity compared with that of keratin-Cu3(PO4)2 nanoflowers and individual Fe3O4 MNPs. Remarkably, keratin-nanoflower@Fe3O4 exhibited superior long-term stability to Fe3O4 MNPs under more acidic conditions and favorable reusability. This method has been successfully exploited for the colorimetric determination of glucose in human serum with satisfactory sensitivity and specificity, offering a novel approach for glucose detection.