Sensitive microscopic quantification of surface-bound prion infectivity for the assessment of surgical instrument decontamination procedures

The new cell-based infectivity method presented here avoids partial or possibly total proteinase K digestion of PrP^Sc in samples for greater sensitivity, in addition to low cost, no ethical concerns, and adaptability to detect different prion strains. This method can be used to test cleaning chemistries' efficacy with greater sensitivity than measuring total residual proteins, which may not correlate with residual prion infectivity.