Exosomal RNA Sequencing from Body Fluid Samples

Exosomes are microcapsules released by different types of cells. It contains DNA, RNA, proteins, and other substances. Exosomes spread in body fluids, and their contents can be phagocytosed by other cells and become an important mediator of intercellular communication. Previous studies show that exosomes secreted by cancer cells are involved in tumorigenesis, growth, invasion, and metastasis. With the improvement of technologies for exosome isolation and deep sequencing, we can identify exosomal RNA profiling for further detection of cancer-related biomarkers. In this chapter, we introduce the experiment procedure and bioinformatic pipeline on exosomal RNA sequencing.