丙咪嗪
细胞凋亡
癌症研究
三阴性乳腺癌
流式细胞术
转移
癌症
MTT法
转移性乳腺癌
医学
癌细胞
乳腺癌
生物
内科学
病理
免疫学
生物化学
替代医学
作者
SHENG-KAI CHEN,Wei Lee,Y. Li,Fei‐Ting Hsu,WEI-TING CHEN
标识
DOI:10.21873/anticanres.16469
摘要
Background/Aim: Triple-negative breast cancer (TNBC) is an aggressive and deadly subtype of breast cancer, and there is an urgent need for new therapeutic strategies. The highly metastatic and anti-apoptotic characteristics are known to be the major factors causing uncontrolled growth in TNBC. Imipramine is a tricyclic antidepressant that possesses anti-inflammatory activity and has been reported to inhibit the progression of highly metastatic non-small cell lung cancer. Materials and Methods: This study used MTT assay, apoptosis markers flow cytometry analysis, open-source data analysis, NF-B reporter gene assay, and western blotting to elucidate the effect of imipramine on MDA-MB-231 and 4T1 cells. Results: Imipramine induced caspase-mediated extrinsic and intrinsic apoptosis and was potentially associated with patient overall survival. Furthermore, imipramine suppressed the invasion and migration abilities and the expression of metastasis-associated proteins in TNBC cells. Conclusion: Imipramine effectively suppressed TNBC progression by inducing apoptosis and inhibiting metastasis.
科研通智能强力驱动
Strongly Powered by AbleSci AI