Highly Sensitive Oxytetracycline Detection Using QCM and Molecularly Imprinted Polymers with Deep Eutectic Solvents

This study presents an efficient method for detecting oxytetracycline, which is critical in environmental monitoring and food safety. A highly sensitive detection platform was developed by combining molecularly imprinted polymers (MIPs) with silica as a carrier, modified with deep eutectic solvents (DES), and a quartz crystal microbalance (QCM) sensor. The MIPs were specifically designed to target oxytetracycline hydrochloride, using SiO2 as the carrier, DES as the functional monomer, N, N-methylenebisacrylamide as the crosslinker, and ammonium persulfate as the initiator. The MIPs exhibited an adsorption capacity of 27.23 mg/g for oxytetracycline hydrochloride. After modification of the MIPs onto a gold electrode surface, a QCM-based sensor platform was constructed. The sensor demonstrated an exceptionally low detection limit of 0.019 ng/mL for oxytetracycline and exhibited excellent sensitivity in tap water. Furthermore, the sensor maintained over 90% detection performance after two weeks of room-temperature storage, indicating its stability. This method provides a rapid, highly sensitive approach for oxytetracycline detection, with potential for future improvements and widespread application in antibiotic testing.