生物传感器
基质(化学分析)
吸附
膜
水溶液中的金属离子
化学工程
化学
有机硅
催化作用
扫描电子显微镜
生化需氧量
微生物
金属
材料科学
细菌
核化学
色谱法
化学需氧量
纳米技术
有机化学
废水
复合材料
废物管理
生物化学
遗传学
工程类
生物
作者
Olga A. Kamanina,Elizaveta A. Lantsova,Pavel V. Rybochkin,V. A. Arlyapov,Yu. V. Plekhanova,A. N. Reshetilov
出处
期刊:Membranes
[MDPI AG]
日期:2022-10-10
卷期号:12 (10): 983-983
被引量:5
标识
DOI:10.3390/membranes12100983
摘要
Biomembranes based on an organosilica sol-gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The use of scanning electron microscopy (SEM) and the low-temperature nitrogen adsorption method (BET) showed that the sol-gel matrix forms a capsule around microorganisms that does not prevent the exchange of substrates and waste products of bacteria to the cells. The use of DEDMS as part of the matrix made it possible to increase the sensitivity coefficient of the biosensor for determining BOD by two orders of magnitude compared to a biosensor based on methyltriethoxysilane (MTES). Additionally, the long-term stability of the bioreceptor increased to 68 days. The use of such a matrix neutralized the effect of heavy metal ions on the microorganisms' catalytic activity in the biosensor. The developed biosensor was used to analyze water samples from water sources in the Tula region (Russia).
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