In Vivo Detection of Double-Stranded RNA by dRBFC Assay

Double-stranded RNA (dsRNA) is the genomic material or replicate intermediate of RNA viruses, and is also a crucial signal molecule to trigger innate immune response and RNA silencing in eukaryotic organisms. Studying the subcellular localization and dynamic of dsRNA provides significant information for understanding RNA virus replication, host responses to virus infection, and viral diagnosis. Several antibody-dependent or -independent methods have been developed to in vivo or in vitro visualize dsRNA in cells. Here, we provide a step-by-step protocol for efficiently visualizing the distribution and dynamics of dsRNA in living plant cells.