硅烷化
基质(水族馆)
葡萄糖氧化酶
X射线光电子能谱
图层(电子)
化学
氧化物
戊二醛
薄膜
化学工程
固定化酶
材料科学
接触角
分析化学(期刊)
生物传感器
纳米技术
有机化学
酶
复合材料
工程类
地质学
海洋学
作者
Sebania Libertino,Filippo Giannazzo,Venera Aiello,Antonino Scandurra,Fulvia Sinatra,Marcella Renis,Marco Fichera
出处
期刊:Langmuir
[American Chemical Society]
日期:2008-01-19
卷期号:24 (5): 1965-1972
被引量:72
摘要
A process to immobilize the enzyme glucose oxidase on SiO2 surfaces for the realization of integrated microbiosensors was developed. The sample characterization was performed by monitoring, step by step, oxide activation, silanization, linker molecule (glutaraldehyde) deposition, and enzyme immobilization by means of XPS, AFM, and contact angle measurements. The control of the environment during the procedure, to prevent silane polymerization, and the use of oxide activation to obtain a uniform enzyme layer are issues of crucial importance. The correct protocol application gives a uniform layer of the linker molecule and the maximum sample surface coverage. This result is fundamental for maximizing the enzyme bonding sites on the sample surface and achieving the maximum surface coverage. Thin SiO2 layers thermally grown on a Si substrate were used. The XPS Si 2p signal of the substrate was monitored during immobilization. Such a signal is not completely shielded by the thin oxide layer and it is fully suppressed after the completion of the whole protocol. A power spectral density analysis on the AFM measurements showed the crucial role of both the oxide activation and the intermediate steps (silanization and linker molecule deposition) to obtain uniform immobilized enzyme coverage. Finally, enzymatic activity measurements confirmed the suitability of the optimized protocol.
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