Preparation and Characterization of Glucose Liposome by Supercritical Reverse Phase Evaporation Method

Liposome has proven a promising carrier for its special structure and characterization, and can be widely used in pharmacy and cosmetics. Supercritical reverse phase evaporation (SRPE) is a new technique for preparation liposomes with supercritical fluid instead of organic solvent. It features that the preparation process is simple and a high coating ratio is obtained for water-soluble substances. In this paper, the technique was used to synthesize liposomes with water soluble glucose and the effects of the processing technologies parameters on the particle size and the coating ratio of liposomes which were investigated in detail. The liposomes with a volume average diameter of 290-330nm were successfully prepared by this technique and the coating ratio for water-soluble glucose was as high as 27.3%, while it was only 12.5% by the process of physical dispersant in water. It was confirmed that the temperature, pressure and equilibrium time had great effects on the particle size and the coating ratio. As the temperature increased from 35°C to 65°C, the particle size decreased obviously to its minimum value and then changed begins to increase . With the pressure increased from 10MPa to 30MPa, the particle size first decreased and then kept approximately constant. As the equilibrium time increased from 15min to 45min, the particle size first kept an approximately constant value and then increased greatly. Increasing either the temperature from 35°C to 65°C or the pressure from 10MPa to 20MPa, the coating ratio increased remarkably, but it decreased as the pressure increased from 20MPa to 25MPa.