槲皮素
壳聚糖
抗氧化剂
生物相容性
化学
纳米颗粒
脂质过氧化
抗坏血酸
体内
氧化应激
肝保护
生物化学
药理学
纳米技术
食品科学
谷胱甘肽
有机化学
材料科学
医学
生物
生物技术
酶
作者
Denitsa Aluani,Virginia Tzankova,Magdalena Kondeva-Burdina,Yordan Yordanov,Elena Nikolova,Feodor Odzhakov,Alexander Apostolov,Tzvetanka Markova,Krassimira Yoncheva
标识
DOI:10.1016/j.ijbiomac.2017.05.062
摘要
The present study deals with development and evaluation of the safety profile of chitosan/alginate nanoparticles as a platform for delivery of a natural antioxidant quercetin. The nanoparticles were prepared by varying the ratios between both biopolymers giving different size and charge of the formulations. The biocompatibility was explored in vitro in cells from different origin: cultivated HepG2 cells, isolated primary rat hepatocytes, isolated murine spleen lymphocytes and macrophages. In vivo toxicological evaluation was performed after repeated 14-day oral administration to rats. The study revealed that chitosan/alginate nanoparticles did not change body weight, the relative weight of rat livers, liver histology, hematology and biochemical parameters. The protective effects of quercetin-loaded nanoparticles were investigated in the models of iron/ascorbic acid (Fe2+/AA) induced lipid peroxidation in microsomes and tert-butyl hydroperoxide oxidative stress in isolated rat hepatocytes. Interesting finding was that the empty chitosan/alginate nanoparticles possessed protective activity themselves. The antioxidant effects of quercetin loaded into the nanoparticles formulated with higher concentration of chitosan were superior compared to quercetin encapsulated in nanoparticles with higher amount of sodium alginate. In conclusion, chitosan/alginate nanoparticles can be considered appropriate carrier for quercetin, combining safety profile and improved protective activity of the encapsulated antioxidant.
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