原位杂交
免疫荧光
原位
分子生物学
生物
化学
信使核糖核酸
抗体
生物化学
遗传学
基因
有机化学
作者
Anushka Dikshit,Hailing Zong,Courtney Anderson,Bingqing Zhang,Xiao‐Jun Ma
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 301-312
被引量:8
标识
DOI:10.1007/978-1-0716-0623-0_19
摘要
Gene expression analysis is critical to precisely characterize complex tissues and provide insight into a disease condition. Techniques like PCR, sequencing, and northern blotting are highly sensitive and specific but are unable to provide information about spatial positioning of target genes. Visualization of gene expression with a spatial context can be critical in identifying complex milieus in heterogenous tissues like tumors. The RNAscope in situ hybridization (ISH) technology detects target RNA expression with high sensitivity and specificity at single-cell resolution. To understand the cellular cross talk between different cell populations, it is essential to simultaneously study gene and protein expression within a complex tissue. This chapter details combining the RNAscope ISH assay with immunofluorescence (IF) in one protocol to simultaneously visualize gene expression and protein expression in human tumor tissue and mouse brain tissue.
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