腺苷
细胞外
嘌呤能受体
运动前神经元活动
细胞生物学
核苷
生物物理学
嘌呤能信号
神经元
化学
胞吐
受体
神经科学
内吞作用
生物
小泡
腺苷受体
生物化学
兴奋剂
作者
Zhaofa Wu,Yuting Cui,Huan Wang,Kun Song,Zhengwei Yuan,Ao Dong,Hao Wu,Yi Wan,Sunlei Pan,Wanling Peng,Miao Jing,Min Xu,Minmin Luo,Yulong Li
标识
DOI:10.1101/2020.05.04.075564
摘要
Abstract The purinergic signaling molecule adenosine (Ado) modulates many physiological and pathological brain functions,but its spatiotemporal release dynamics in the brain remains largely unknown. We developed a genetically encoded G PC R - A ctivation– B ased Ado sensor (GRAB Ado ) in which Ado-induced changes in the human A 2A receptor are reflected by fluorescence increases. This GRAB Ado revealed that neuronal activity-induced extracellular Ado elevation was due to direct Ado release from somatodendritic regions of the neuron, requiring calcium influx through L-type calcium channels, rather than the degradation of extracellular ATP. The Ado release was slow (∼30 s) and depended on equilibrative nucleoside transporters (ENTs) rather than conventional vesicular release mechanisms. Thus, GRAB Ado reveals an activity-dependent slow Ado release from somatodendritic region of the neuron, potentially serving modulating functions as a retrograde signal.
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