拼接因子
RNA剪接
选择性拼接
癌变
卵巢癌
癌症研究
外显子
基因表达
医学
外显子剪接增强剂
生物
基因
癌症
分子生物学
内科学
遗传学
核糖核酸
作者
Séverine Iborra,Marc Hirschfeld,Markus Jaeger,Axel zur Hausen,Elena Ioana Braicu,Jalid Sehouli,G. Gitsch,Elmar Stickeler
出处
期刊:International Journal of Gynecological Cancer
[BMJ]
日期:2013-07-01
卷期号:23 (6): 990-996
被引量:28
标识
DOI:10.1097/igc.0b013e31829783e3
摘要
Objective
Alternative splicing represents an important nuclear mechanism in the posttranscriptional regulation of gene expression, which is frequently altered during tumorigenesis. Previously, we described marked changes in alternative splicing of the CD44 gene in ovarian and breast cancer as well as specific induction of distinct splicing factors during tumor development. The present study was focused on the expression profiles of different splicing factors, including classical serine-arginine (SR) proteins including ASF/SF2, hTra2β1, hTra2α, and Y-box–binding protein (YB-1) in physiological and malignant epithelial ovarian tissue to evaluate their expression pattern with regard to tumor development and disease progression. Materials and Methods
Expression levels of the different splicing factors were analyzed in physiological epithelial ovarian tissue samples, primary tumors, and metastatic samples of patients with a diagnosis of epithelial ovarian cancer using quantified reverse transcription polymerase chain reaction analysis. We examined more closely the splicing factor hTra2β1 using Western blot analysis and immunohistochemistry. Results
The analysis revealed a marked and specific induction of ASF/SF2, SRp20, hTra2β1, and YB-1 in primary tumors as well as in their metastatic sites. However, in our patient cohort, no induction was seen for the other investigated splicing factors SRp55, SRp40, and hTra2α. Conclusions
Our results suggest a specific induction of distinct splicing factors in ovarian cancer tumorigenesis. The involvement of hTra2β1, YB-1, SRp20, and ASF/SF2 in exon recognition and alternative splicing may be important for gene regulation of alternatively spliced genes like CD44 with potential functional consequences in this tumor type leading to progression and metastasis.
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